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Molekul: Jurnal Ilmiah Kimia

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Published by Universitas Jenderal Soedirman

ISSN : 19079761 EISSN : 25030310 DOI : -

Core Subject : Science, Engineering,

Chemical Engineering, Chemistry & Bioengineering Chemistry

MOLEKUL is a peer-reviewed journal of chemistry published by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Indonesia. Publishing frequency 2 issues per year, on May and November. This Journal encompasses all branches of chemistry and its sub-disciplines including Pharmaceutical, Biological activities of Synthetic Drugs, Environmental Chemistry, Biochemistry, Polymer Chemistry, Petroleum Chemistry, and Agricultural Chemistry.

Arjuna Subject : -

Articles 8 Documents

Search results for , issue "Vol 6, No 1 (2011)" : 8 Documents clear

STUDI PENURUNAN ZAT WARNA ACID ORANGE 7 DENGAN PROSES OKSIDASI MENGGUNAKAN FERRAT (FeO4)2- Dian Windy Dwiasi; Suyata Suyata
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Colour removal from textile wastewater has been a matter of considerable interest during the last two decades, not only because of the potential toxicity of certain dyes but often due to their visibility in receiving waters and to their low biodegradability. Due to the limited success of some physical and chemical techniques for the treatment of dye effluents it is necessary to develop destructive systems leading to complete mineralization or, at least, to less harmful or easy-to-treat compounds. Oxidation processes using ferrate (FeO4)2- have been found to be very effective in the degradation of dye pollutants and for the treatment of waste waters from the textile industry.Acid Orange 7 (AO7) commonly used as a textile dye and could be degraded by ferrate oxidation processes. In the oxidation degradation of dye by ferrate process, effect of some parameters such as time, pH, and molar ratio of dye was examined at experimental condition. AO7 removal by this process was calculated to be equal to 12 minute at experimental condition. Ferrate can oxidize acid orange 7 effectively at optimum pH of 9.8, with the molar ratio of ferrate : acid orange 7 at 4:1. The percentages of acid orange 7 degradation reached to 98.9%.

KARAKTERISASI ABU TERBANG PLTU CILACAP UNTUK MENURUNKAN KESADAHAN AIR DI DESA DARMAKRADENAN KECAMATAN AJIBARANG KABUPATEN BANYUMAS Senny Widyaningsih; Ely Setiawan; Tien Setyaningtyas
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Fly ash is an ash with smooth granular shape and black grey color. It is a waste material produced from the burning of coal. The contain of fly ash especially is silica and alumina that it can be used as adsorbent. As adsorbent, fly ash as used to decrease water hardness at Darmakradenan Village, Ajibarang District. The result showed that the porosity of fly ash was 13.6056%, the water content was 0.055%, adsorption capacity of iodium was 216.8975 mg/g, and adsorption capacity of methylen blue was 0,3891 mg/g. Fly ash could decrease total water hardness. The adsorption rise when contact time was added. Adsorption reached equilibrium at contact time 120 minutes with decreasion percentage 63.6363%.

PENGARUH SIANIDA PADA FOTOREDUKSI Hg(II) YANG DIKATALISIS TiO2 Diky Hidayat
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Influence of cyanide on the photoreduction of Hg(II) catalyzed by TiO2 had been studied. Photoreduction process was carried out in a closed reactor equipped with UV lamp The process was done by radiated and stirred a mixture of Hg(II) solution, TiO2, and cyanide for a certain period of time. The amount of reduced Hg(II) by photoreduction was calculated by the substracting initial Hg(II) concentration with unreduced one, which was determined by usedcold vapor atomic absorption spectrophotometer method. In this research influences of solution concentration and pH solution in the presence cyanide were also evaluated.The result of this research indicated that the presence of cyanide lead to decrease Hg(II) photoreduction, and a drastical drop was observed when more than 2 mmole cyanide was added for each mmole of Hg(II). Increased pH solution from 2 to 4 in the presence of cyanide can improve photoreduction process, but further increased of pH solution higher than 4 lead to decrease photoreduction of Hg(II).

SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL Diana Indrasanti; Aris Haryanto; Wayan T. Artama
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Microneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC-3) is the protein that plays an important role in the invasion proccess during cell infection as a mediator attachment parasite to the host cell. The aim of this research is to clone mic3 (gene encoding for MIC-3) of T. gondii from local isolate using recombinant DNA technology by cloning mic3 in an expression vector. Deoxyribonucleic acid (DNA) from T. gondii tachyzoites was amplified by PuRe Taq RTG-PCR Beads using mic3 specific primers. Amplified DNA was double digested using EcoRV and HindIII restriction endonucleases and then purified using EZ-10 spin coloumn purification kit. The mic3 DNA was ligated into pET-32a(+) expression vector and transformated into Escherichia coli BL21. The results showed that recombinant mic3gene 4.2 kDa has been successfully performed by cloning gene encoding for MIC-3 protein of T. gondii local isolate into pET-32a(+) and transformed to E. coli BL21.

ISOLASI DAN KARAKTERISASI PROTEASE ALKALIN DARI ISOLAT BAKTERI LIMBAH TERNAK DI EXFARM FAKULTAS PETERNAKAN UNSOED Zusfahair Zusfahair; Puji Lestari; Ari Asnani
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Protease is one of the widely used enzymes for the industry. The potential resource of microorganism that produced protease is milk cow waste. In this research, isolation and characterization has been done toward isolated protease from milk cow waste of the Exfarm’s Animal Husbandry Faculty at University of Jenderal Soedirman, Purwokerto. The research used experiment method and the parameters observed were the genus of bacteria which produce protease and the activity of protease. The characterizations of protease were determination of optimum pH and temperature, the influence of metal ions, EDTA, surfactant, and commercial detergent toward enzyme activity, and also the study of enzyme stability. The results from the research showed that the isolated bacteria from the Exfarm’s of Animal Husbandry Faculty of UNSOED, which produced protease was Salmonella sp. Characterization of isolated Salmonella sp. from 45% ammonium sulphate fraction indicated that the optimum temperature was 50 ºC, optimum pH was 8, the enzyme was activated by Ca2+ dan Mg2+ ion, whereas it was inhibited by Zn2+, Cu2+ ions and EDTA. The addition of Tween-80 with the concentration of 0.2% and 0.4% increased protease activity, however the addition of Tween-80 with concentration higher than 0.6% decreased the protease activity. Enzyme protease from isolated Salmonella sp. was relatively stable with the addition of commercial detergent such as Attack, Surf, and Bukrim.

SINTESIS DAN KARAKTERISASI CROSSLINK KITOSAN DENGAN TRIPOLIFOSFAT pH 3 Mardiyah Kurniasih; Nurul Hidayat Aprilita; Indriana Kartini
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Chitosan is polycationic in acidic media and can interact with negatively charged species such as tripolyphosphate (TPP), which is a nontoxic crosslinking agent. The synthesis of TPP crosslinked chitosan was perfomed by reacting TPP solution (pH 3) with chitosan solution. The aim of this study was to prepared and characterize the crosslinked chitosan-tripolyphosphate samples. Characterization included determination of water and ash degree, moreover crosslinked chitosan-tripolyphosphate powder characterize with FTIR and XRD spectroscopy. The result showed that process efficiency of crosslinked chitosan-tripolyphosphate was 88.49%, with degree of water and ash were 25.70 and 16.72%, respectively.

KONSTRUKSI MUTAN PROTEIN FOSFATASE ptc2D Saccharomyces cerevisiae DENGAN METODE PENGGANTIAN GEN TARGET DENGAN POLYMERASE CHAIN REACTION (PCR) Hermansyah Hermansyah
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Yeast Saccharomyces cerevisiae is an excellent model to studi genes function of eukarotic cells such as study of gene encoding protein phosphatase PTC2. Novel phenotypic caused by mutated gene is an important step to study function of gene. In this study constructed mutant of PTC2 gene encoding protein phosphatase. Method that used in this construction was replacement of target gene (PTC2) with auxotroph marker Candida albicans HIS3 by Polymer Chain Reaction (PCR) or called by PCR-mediated disruption. Mutant colonies which grew in selective medium SC without histidine were confirmed by PCR amplification. By using 1% Agarose gel electrophoresis the result showed that size of ptc2D::CgHIS3 transformant was 3.52 kb while wild type strain was 2.9 kb, indicated thatptc2D::CgHIS3 has integrated on chromosome V replacing PTC2 wild type.

EFEKTIVITAS ECENG GONDOK (Echhornia crassipes) DALAM PENYERAPAN KADMIUM (Cd) PADA LEACHATE TPA GUNUNG TUGEL Sri Lestari; Slamet Santoso; Sulastri Anggorowati
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Leachate is liquid of result organic garbage that contain substance is dissolved and suspension as decomposition by microorganism. Leachate contain material organic with high level and many formed at landfill with open dumping system. Material organic in leachate can be reduced by phytoremediation with water hyacinth (Echhorniacrassipes). The aim of research are know large closure of water hyacinth, long residence time and the best interaction to reduce the levels of material organic in leachate. Method of research was designed experimental based on Completely Randomized Design (CRD) with Factorial Design. The first factor are closing large percentage of water hyacinth (0%, 25%, 50%, 75% and 100%) and the second factor are long residence time (4 days, 5 days and 6 days). Result of research showed that is closing large of 75% is the best treatment to reducing level of Cd that is 29,279%. and long residence time of 6 days is the best treatment to reducing level of Cd that is 27,211%. Interaction closing large of 75% and long residence time of 6 days is the best treatment to reducing level of Cd that is 39,770%

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Contact Name
Amin Fatoni

Contact Email
aminfatoni@unsoed.ac.id

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Journal Mail Official
j.molekul@gmail.com

Editorial Address
Jl. Dr. Soeparno No.61 Karangwangkal, Purwokerto, Jawa Tengah 53

Location
Kab. banyumas,

Jawa tengah

INDONESIA

Abstract

Abstract

Abstract

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Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Amin Fatoni

Contact Email aminfatoni@unsoed.ac.id

Phone -

Journal Mail Official j.molekul@gmail.com

Editorial Address Jl. Dr. Soeparno No.61 Karangwangkal, Purwokerto, Jawa Tengah 53

Location Kab. banyumas, Jawa tengah INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Amin Fatoni

Contact Email
aminfatoni@unsoed.ac.id

Phone
-

Journal Mail Official
j.molekul@gmail.com

Editorial Address
Jl. Dr. Soeparno No.61 Karangwangkal, Purwokerto, Jawa Tengah 53

Location
Kab. banyumas,

Jawa tengah

INDONESIA